Development and Validation of qPCR Methods for the Determination of Kudoa thyrsites Infection Levels

Description

The development and validation of the qPCR technique will provide industry with an early, quantitative detection method for K. thyrsites, and will be invaluable as a method of monitoring populations prior to harvest to establish K. thyrsites infection levels and potential for tissue damage. Such information is essential to the producer in determining how the fish will be processed and sold. Quantitative PCR will also allow rapid assessment of treatment effects in terms of host susceptibilities to K. thyrsites. In doing so, qPCR will provide the means of rapidly testing the efficacy of vaccine candidates for K. thyrsites.

Program Name

Aquaculture Collaborative Research and Development Program (ACRDP)

Year(s)

2004 - 2006

Ecoregion(s)

National

Principal Investigator(s)

Kristi Miller
Email: MillerK@pac.dfo-mpo.gc.ca